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The paper “Model-based pre-processing in Raman spectroscopy of biological samples”, authored by Kristian Hovde Liland, Achim Kohler and Nils Kristian Afseth has been published in Journal of Raman Spectroscopy.

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Abstract

Modelbased pre-processing has become wide spread in spectroscopy and is the standard procedure in Fourier-transform infrarespectroscopy. It has also been shown to give valuable contributions in Raman spectroscopy. Extended multiplicative signal correction is flexible enough to handle varying fluorescence background and take into account individual variations in baselines whilstill keeping enough rigidity through reference spectra and model fitting to avoid degenerate solutions and overfitting, when used correctly. We demonstrate the basic extended multiplicative signal correction method and some extensions, including a novel shifcorrection, on real Raman data to demonstrate effects on visual appearance, replicate variation and prediction. Comparisons with other standard correction methods are also shown and discussed.

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